Amphizonella violacea, c. 230 µm long, feeding on algae

 

 

Genus Amphizonella Greeff, 1866

 

Diagnosis: body more or less spherical, surrounded by a flexible, bilayered pellicle; outer layer gelatinous, 8-12 µm thick with fine denticles, inner layer thin, chitinoid, undulates with internal movements, sac-like; pseudostome invaginated and variable; pseudopodia cylindrical, finely granular with rounded ends; movement slow; endoplasm clear, violet, yellow granules in purple vesicles; one or more ovular or spherical nuclei; the nuclear membrane is easily visible, the numerous nucleoli are mainly concentrated below the nuclear membrane. Contractile vacuoles 20-30 µm; no crystals; cysts reported.

One known species:

 

Amphizonella violacea Greeff, 1866

 

Diagnosis: as for the genus.

Non moving cells are usually roundish or oval, but can emit pseudopods. Moving amoebae adopt a more longish tongue or fan-like shape. Sometimes non moving amoebae take a stretched form with pseudopods at opposite ends. In contrast to previously published images the outer gelatinelike mucus layer is not always present. After excystation this layer is secreted de novo. Its thickness is variable and can reach up to 23 µm. Normally it contains numerous rod-shaped bacterial exobionts of unknown function. In SEM the outer surface is smooth. The untextured organic shell-wall is flexible and opens in a large not well defined aperture.

 

Dimensions: literature 125-250 µm, nucleus up to 40 µm (Penard, 1906); Meisterfeld (2006): 75-558 µm. My measurements: 198-264 µm, nucleus 39.1-41.7 µm (n=8).

 

Ecology: xerophilic mosses on roofs. Algivorous. I found this species between algae in a shallow part of a freshwater pond in the early spring of 2015 (see Crailoo).

 

Remarks: Meisterfeld and Badewitz (2006) collected this conspicuous, but often overlooked species, in xerophilic mosses from roofs in six localities in Germany. They found the overall shape much more variable than hitherto known and in one population of small specimen the violet color was lacking. The observed cells were uninucleate. Penard (1902) also observed one ovular nucleus per cell, but occasionally he found two or three nuclei in a cell.

 

Literature: Meisterfeld and Badewitz, 2006.

 

Amphizonella violacea, shortly after division
Amphizonella violacea, with nucleus located centrally.
Amphizonella violacea,  engulfing some algae.
Amphizonella violacea, nuclei.
 
A. violacea,, mucus layer arrowed

 

Two filamentous algae being engulfed by the amoeba.
Specimen which divided; the other cell moved away leaving the mucus layer behind.
Amphizonella violacea, c. 230 µm long