Limnofila cf oxoniensis

 

 

Limnofila oxoniensis Bass and Cavalier-Smith, 2009

 

Diagnosis: cell bodies roughly elliptical or often irregular with polygonal outline; often in pairs or small clusters, or aligned longitudinally, connected (or apparently so) via filopodia; extremely slender filopodia extend from the cell often most prominently from the longitudinal apices, sometimes radiate more evenly though not so regularly as to confer radial symmetry. Filopodia several to 10X body length; attached to substrate; often branched, sometimes apparently anastomosing, granular, often heavily; granules often motionless but can move slowly along them. Filopodia decrease in thickness and visibility within creasing distance from the cell body; distally 0.1 mm wide, visible only under excellent optics; movement of cell bodies across substrate very rare or indiscernible. No flagellate phase or cysts.

 

Dimensions: cell bodies 7-25 µm.

 

Ecology: Bacterivorous. Type locality Oxford garden soil. I find this species sometimes in wet mounts kept in a moist chamber. I have found it in sediments from several locations in the Netherlands.

 

Remarks: Differs from L. mylnikovi in forming looser clusters or isolated cells not always connected by cytoplasmic strands; cell bodies usually much larger; filopodial anastomoses more common.

 

The cells in the photomicrographs on this page were present in moist chamber preparations from freshwater sediments. They had long branching granulofilopodia, and the cytoplasm in the body was constantly turning and moving.

 

Limnofila cf oxoniensis

Limnofila cf oxoniensis  freshwater, Laegieskamp, the Netherlands

Limnofila cf oxoniensis  freshwater, Laegieskamp, the Netherlands

Limnofila cf oxoniensis  freshwater, Laegieskamp, the Netherlands

Limnofila cf oxoniensis  freshwater, Laegieskamp, the Netherlands

Limnofila cf oxoniensis: Two cells with long granulated filopodia

These cells were photographed one day after the first picture above. One cell has divided.

Four cell s with a number of granulopodia (also see the detail below)

The same cells as in the photomicrograph above

Limnofila oxoniensis

Three cells, each body about 8 µm long, with long branching granulopodia in contact with the substrate.

Limnofila oxoniensis

Compare the filopodia with those on the first photomicrograph; they are contracted or stretched. Time between both photos 15 seconds.

Limnofila oxoniensis

The same cells in normal illumination. No relevant details as nucleus and crystals were visible.