Lieberkuehnia wageneri
Lieberkuehnia wageneri, note the relatively thick organic-walled test. This specimen was collected by Kenneth Dumack in 2017 from a new fishpond in Germany. The sample was scraped from black plastic foil on the bottom of the pond.

 

 

Lieberkuehnia wageneri Claparède et Lachmann, 1859

 

Diagnosis: Cell fragile, extremely variable in size, 50-(80-120)-200 µm long, with an organic-walled test with a single aperture. Test in general sub-spherical to ovoid or pyriform, sometimes elongated pyriform. Test hyaline, colourless or slightly yellowish, very supple and deformable, smooth and delicate. The anterior part of the cell tapers towards the eccentric, oblique and crescent shaped aperture.  Where the eccentric pseudopodial peduncle reaches the aperture, a vast network of branching pseudopodia radiates. Protoplasm clear, in continuous streaming,  with many small granules, bi-refringent granules and globules, vacuoles and nuclei. The amount of nuclei may strongly differ per cell, but is always considerable, seldom fewer than 30 and often more than 150. Nuclei spherical, commonly 6 µm in diameter and each nucleus with one hemispherical nucleolus, laying close to the nuclear membrane. In specimens with a low amount of nuclei the diameter of the nuclei is larger, up to 12  µm. Rarely there are two or three nucleoli present, and large nuclei can contain up to six biconvex shaped nucleoli, all close to the nuclear membrane.  

 

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Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri - cell strongly compressed and damaged, the test is c. 3-4 µm thick
Lieberkuehnia wageneri
Lieberkuehnia wageneri - nuclei
Lieberkuehnia wageneri
Large cell with pseudopodial peduncle (p)
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Lieberkuehnia wageneri
Crescent shaped aperture