Genus Limnofila Cavalier-Smith and Bass, 2009
Diagnosis: trophic phase a small, often globular cell; cilia if present generally not visible in the light microscope; with branched regularly granular and very slender, exceedingly thin thread-like filopodia. Bacterivorous. Freshwater.
Type species Limnofila borokensis Cavalier-Smith and Brass, 2009.
Remarks: ultrastructurally studied species (borokensis and mylnikovi) have flat mitochondrial cristae, bundles of 2€”6 microtubules supporting the filopodia, complex concentric-structured extrusomes, two ciliary stubs with transverse plate at the base of the transitional region but no 9+2 axonemes, no obvious paranuclear microbody, no prominent centrosome (unlike Heliomonadida), and no large granules in the distal centriolar matrix. Nuclear division closed pleuromitosis with extranuclear spindle.
Limnofila mylnikovi Cavalier-Smith and Bass, 2009
Diagnosis: cell body 5-6.6 µm, branched filopodia 1-4 x body length; unlike L. borokensis lacks swimming biflagellate stage or mobile lobopodia-driven amoeboid stage and cysts in cultures; single contractile vacuole; forms cell clusters in rows.
